Accelerate your high resolution HLA typing.

HLA class I are fully compatible with our powerful SBTengine® analysis software.
With excellent exon coverage we can solve practically any ambiguity, allelic ambiguities by sequencing additional exon regions and genotypic ambiguities with Group Specific Sequencing Primers (GSSP).

The assay set-up provides an efficient combination of generic and Group Specific Sequencing Primers (GSSPs) to accomplish enhanced ambiguity resolution in one single round of sequencing.

Your benefits:

One PCR, Complete gene amplification in a single PCR.
Analysis of essential regions exon 2, 3 and 4 possible.
Enhanced allele ambiguity resolution. Complete exon 2, 3 and 4 sequence information

Accelerate your high resolution HLA typing.

HLA class I are fully compatible with our powerful SBTengine® analysis software. With excellent exon coverage we can solve practically any ambiguity, allelic ambiguities by sequencing additional exon regions and genotypic ambiguities with Group Specific Sequencing Primers (GSSP).

Your benefits:

One PCR, Complete gene amplification in a single PCR.
Analysis of essential regions exon 2, 3 and 4 possible.
Enhanced allele ambiguity resolution. Complete exon 1-5 sequence information.

Accelerate your high resolution HLA typing.

The HLA-DRB1 kit includes an amplification and sequencing strategy which enables amplification and sequencing of HLA-DRB1 exons 2 and 3.

For convenience, a codon 86–specific sequencing primer R8 is included. This primer may either be routinely used for sequencing or used when needed after the initial sequencing with the regular sequencing primers.

Your benefits:

One PCR, Exon 2 to 4 amplified in a single multiplexed PCR.
Splitting DR groups will result in lower number of ambiguities in first round of sequencing.
Analysis of essential regions exon 2-3 possible.
Enhanced allele ambiguity resolution. Complete exon 2 and exon 3 sequence information available.
Codon 86-specific sequencing primer (R8) included.
Enhanced allele ambiguity resolution. Complete exon 2 and exon 3 sequence information available.

Accelerate your high resolution HLA typing.

The HLA-DQB1 kit includes a redesigned amplification and sequencing strategy which enables enhanced sequencing of HLA-DQB1 exons 2 and 3.

For HLA-DQB1 Exon 1 to 4 is amplified in a single multiplexed PCR Subsequently both alleles are sequenced simultaneously. If required, group specific sequencing primers (GSSP) may be used to solve genotype ambiguities. A GSSP will generate a homozygous sequence of one of the alleles only.

Your benefits:

One PCR, Exon 2. 3 & 4 amplified in a single multiplexed PCR.
Analysis of essential regions exon 2 & 3 possible.
Enhanced allele ambiguity resolution. Complete exon 2 and exon 3 sequence information available.

Accelerate your high resolution HLA typing.

For HLA-DPB1 Exon 1 to 5 is amplified in a single multiplexed PCR. Subsequently both alleles are sequenced simultaneously. If required, group specific sequencing primers (GSSP) may be used to solve genotype ambiguities. A GSSP will generate a homozygous sequence of one of the alleles only.

Your benefits:

One PCR, Exon1 to 5 amplified in a single multiplexed PCR.
Analysis of essential regions exon 2 and exon 3 possible.
Enhanced allele ambiguity resolution. Complete exon 1 and exon 4 sequence information available.

Accelerate your high resolution HLA typing.

For HLA-DPA1 Exon 1 to 4 is amplified in a single multiplexed PCR Subsequently both alleles are sequenced simultaneously. If required, group specific sequencing primers (GSSP) may be used to solve genotype ambiguities. A GSSP will generate a homozygous sequence of one of the alleles only.

Your benefits:

One PCR, Exon1 to 4 amplified in a multiplexed PCR.
Analysis of essential regions exon 2 possible.
Enhanced allele ambiguity resolution. Complete exon 1 and exon 4 sequence information available.

Accelerate your high resolution HLA typing.

For HLA-DQA1 Exon 1 to 4 is amplified in a single multiplexed PCR Subsequently both alleles are sequenced simultaneously. If required, group specific sequencing primers (GSSP) may be used to solve genotype ambiguities. A GSSP will generate a homozygous sequence of one of the alleles only.

Your benefits:

One PCR, Exon1 to 5 amplified in a single multiplexed PCR.
Analysis of essential regions exon 1-4 possible.
Enhanced allele ambiguity resolution. Complete exon 1 and exon 4 sequence information available.

Accelerate your high resolution HLA typing.

The HLA-DRB 3/4/5 Core kit includes 3 amplification primer sets (i.e. HLA-DRB3, HLA-DRB4 and HLA-DRB5). The sequencing strategy enables complete sequencing of the HLA-DRB 3/4/5 exons 2 and 3 with general sequencing primers. The codon 86 GSSP enables you to solve the most frequent ambiguities.

Your benefits:

One PCR, Exon 2 to 4 amplified in a single multiplexed PCR.
Codon 86-specific sequencing primer (R8) included.
Enhanced allele ambiguity resolution. Complete exon 2 and exon 3 sequence information available.

First commercially available SBT HLA-G typing kit

SBTexcellerator® HLA-G brings fast and easy high resolution HLA-G Sequencing-Based Typing (SBT) within reach.

The SBTexcellerator® HLA-G kit is a novel HLA-G assay that allows high level of sequencing coverage and genotyping resolution. HLA-G is found to play a role in immune tolerance in pregnancy and is expressed in the placenta.

More research is being done to further explore its role in other mechanisms and diseases like HIV and tumor development.

Your benefits:

SBTexcellerator^® strategy for high-resolution HLA-G genotyping. The forward and reverse amplification primers (red arrows) amplify exon 1 up to exon 6 in one single long-range PCR. The exon 2, 3 and 4 sequencing primers (yellow arrows no. 3 to 8) are used for core sequencing, while exon 1 and 5 primers (yellow arrows no. 9 to 12) are for sequencing of the extended regions in case allele ambiguities need to be resolved.

High resolution HLA-G genotyping
One PCR, Exon 1 to 6 amplified in a single multiplexed PCR.
bi-directional sequencing of complete exon 2-4 possible.
Enhanced ambiguity resolving power due to Bi-directional analysis of exon 1 and exon 5