Now equipped with rehydration and focusing trays, the redesigned PHERO-iefoc has been
optimised to perform first-dimension isoelectric focusing (IEF) with IPG (immobilised pH
gradient) strips quickly, easily and reproducibly. An ideal entry-level system for both
inexperienced and occasional IEF users, the PHERO-iefoc is also versatile enough to
meet the needs of laboratories with increased throughput requirements.
Features include:
– A high-capacity focusing tray that accommodates up to twelve IPG strips
Adjustable ‘pick-and-place’ electrodes clip conveniently anywhere within the focusing ray to resolve IPG strips 7-24cm in length; colour-coded to prevent polarity reversal
– A cooling plate, manufactured from a special grade ceramic in a large 26x26cm surface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high voltage IEF techniques
– An optional, but recommended recirculating chiller connects quickly and easily to the cooling plate via snap-lock connectors to maintain optimal operating temperatures for IPG strips (20°C) and precast gels (4°C)
– Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF to maximise throughput, and provides useful storage at -20°C for focused strips before second-dimension runs
– Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels